Abstract
A magnetite mine drainage sample collected from Han-Xing Iron mine area in Hebei Province was domesticated with potassium dichromate. Four kinds of media supplemented with 300mg/L chromium(VI) were used to isolate chromium(VI) tolerant bacteria. 10 Cr(VI) reducing strains which can tolerant at least 350 mg/L Cr(VI) and reduce the Cr(VI) efficently are screened by observing the growth station and measuring the remaining chromium (VI) concentration in media. 16S rDNA sequencing and phylogenetic analysis showed that Brevundimonas spp. are the main group of chromium(VI) tolerant bacteria in the magnetite mine drainage water sample.
Highlights
A magnetite mine drainage sample collected from Han-Xing Iron mine area in Hebei Province was domesticated with potassium dichromate
We found there are a lot of chromium (VI) tolerant bacteria in the magnetite mine drainage samples
The chromium tolerant bacteria in the sample were domesticated by the following procedure: the mine drainage www.ccsenet.org/mas sample was inoculated by 5%(v/v) to the domestication medium supplemented with 200 mg/L of potassium dichromate, cultured for 3 days at 28 °C by shaking at 200r/min; the domesticated sample was transferred by 5%(v/v) to a new domestication medium supplemented with 400 mg/L of potassium dichromate, culturing for 3 days by the same condition; and the like, until the potassium dichromate concentration up to 1000 mg/L for domestication culture
Summary
A magnetite mine drainage sample collected from Han-Xing Iron mine area in Hebei Province was domesticated with potassium dichromate. Four kinds of media supplemented with 300mg/L chromium(VI) were used to isolate chromium(VI) tolerant bacteria. 10 Cr(VI) reducing strains which can tolerant at least 350 mg/L Cr(VI) and reduce the Cr(VI) efficently are screened by observing the growth station and measuring the remaining chromium (VI) concentration in media. 16S rDNA sequencing and phylogenetic analysis showed that Brevundimonas spp. are the main group of chromium(VI) tolerant bacteria in the magnetite mine drainage water sample. We found there are a lot of chromium (VI) tolerant bacteria in the magnetite mine drainage samples. We tried to isolate the Cr(VI) reducing bacteria from a magnetite mine drainage sample and determine the phylogenetic status of them
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