Abstract
Two isolectins have been isolated from Lathyrus ochrus (L.) DC. seeds by ammonium sulfate precipitation, affinity chromatography on Sephadex G-100 and subsequent chromatofocusing. The isolectins, whose isoelectric points are respectively pH 7.2 and pH 6.0, have a relative molecular mass about 49 000 and are tetramers consisting of two slightly different kinds of light (Mr 4500) and heavy (Mr 20 000) subunits. The amino acid composition, N-terminal amino acids, carbohydrate and metal content of both the isolectins and their subunits have been compared. The isolectins are non-specific in hemagglutination and agglutinate equally well human erythrocytes of different ABO groups. Their hemagglutinating activity is inhibited best by D-mannose, D-glucose and their α-methylglucosides derivatives. They share antigenic determinants in common with other Lathyrus lectins and other lectins belonging to the Vicieae tribe.
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