Isolation and partial characterization of serum immunoglobuline in two species of sturgeons, Siberian sturgeon (Acipenser baerii) and Stellate sturgeon (Acipenser stellatus)

Abstract

The immunoglobulins (Igs) were isolated and purified respectively from serum of Siberian sturgeon (Acipenser baerii) and Stellate sturgeon (Acipenser stellatus) by using 50% saturated ammonium sulfate precipitation. Analysis of the purified Ig of the two species of fishes on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions revealed two bands, heavy chains and light chains respectively. The molecular weight of the heavy chains of two fishes was about 70 kD and the molecular weight of the light chains was 27kD for Siberian sturgeon and 27.5 kD for Stellate sturgeon. The results suggested that the isolated and purified serum Immunoglobuline in two species of sturgeons, Siberian sturgeon (A. baerii) and Stellate sturgeon (A. stellatus) can be used to produce polyclonal and monoclonal antibodies against sturgeon Ig for developing diagnostic tests against a wide variety of pathogens.