Abstract
The study was conducted to isolate and identify cellulose producing microorganisms from cassava waste water. Cassava waste water sample was obtained from a cassava processing factory at Lokogoma town in Wushishi Local government area of Niger State. The microorganisms were isolated, identified and counted by standard microbiological methods. The mean bacteria count ranges from 6.8 x 103 cfu/mL-1 to 2.1 x 103 cfu/mL-1 while the fungi count ranges from 3.2 x 103 cfu/mL-1 to 1.2 x 103 cfu/mL-1. A total of eight (8) bacterial; Staphylococcus aureus, Bacillus anthrax, Bacillus subtilis, Escherichia coli, Klebsilla sp, Bacillus megaterus, Staph. Epidermidis and Pseudomonas aeruginosa, and six (6) fungi; Saccharomyce serivicea, Aspergillus niger, Penecillium sp., Muccor sp., Aspergilus flavus and Aspergilus fumigetus isolates were identified in the waste water. Among these organism, the best cellulase activity was recorded for Bacillus subtilis (10.39 x 10-4 mg/ml/sec) and Aspergillus niger (11.21 x 10-4 mg/ml/sec). However, maximum activity was obtained at pH ranges from 3 ~ 9, temperature ranges from 30 oC ~ 80oC and substrate concentrations ranges from 1.5% ~3.0%. In conclusion, cassava processing water regarded as waste water could be an alternative source of microorganisms capable of producing cellulase enzyme for industrial purposes.
Highlights
Biomolecules derived from natural resources are playing a major role in manufacturing products needed for daily use [1]
Staphylococcus aureus, Bacillus subtilis, Bacillus megaterus and Klebsilla sp. had higher percentage bacterial occurrence of 58.33%, 50.00%, 25% and 25% respectively while (8.33%) Staph
The higher percentage occurred fungal isolates were Saccharomyce serivicea (83.33%), Aspergilus niger (66.66%) and Penecillium sp. (50.00%)
Summary
Biomolecules derived from natural resources are playing a major role in manufacturing products needed for daily use [1]. Enzymes are one of those molecules that are globally recognized for their multifarious applications in industries Their utility in brewing, dairy products, detergents, food and feed, pharmaceutical production, and paper and pulp industry is huge. Cellulose the substrate of cellulase is the most abundant biological compound on terrestrial and aquatic ecosystem, and is the main component of plant biomass [3] It is the dominant waste material from agricultural industry in the form of stalks, stems and husk, and there has been great interest in utilizing cellulose as an energy resource and feed [4]. The enzyme breaks β-1,4-linkages in cellulose polymer to release sugar subunits such as glucose This notion is applied in industries either cellulose is utilized as a raw material or cellulose degradation is a must [6]
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