Isolation and Morphological Identification of Cellulolytic Fungi from Domestic Waste in South Toapaya Village, Bintan Regency

Abstract

Domestic waste is one of the unresolved issues. The main challenge is the difficulty in degrading domestic waste due to its lignocellulosic content, which is hard to break down. One of the uses of cellulolytic fungi is identified as organisms capable of breaking down cellulose components in domestic waste and assisting in speeding up the degradation process. The aim of this research is to discover and identify the types of cellulolytic fungi present in household domestic waste in the South Toapaya Village. This research was conducted by collecting samples in South Toapaya Village and analyzing them at the Provincial Marine Service Laboratory of Riau Province from February to August 2023. The research methods included field exploration, laboratory analysis, identification with reference to the literature, as well as microscopic and macroscopic observations. The research results show that out of 14 fungal isolates isolated from domestic waste, 8 of them have significant cellulolytic activity. Among them, the isolate with code UMRCC 03 has the highest cellulolytic index of 3.24 and is identified as the Aspergillus sp. fungus, while the isolate with code UMRCC 05 has a cellulolytic index of 2.14 and is identified as the Trichoderma sp. Fungus.