Isolation and Molecular Iidentification of Klebsiella Microbe Isolated from Chicks

Abstract

We aim to isolation and identification of klebsiella organisms causing death and loss in chicks, Antibitoic sesetivity test,PCR detection of genus specific primer of klebsiella ,pcr detection of klebsiella carbapenem resistance gene (kpc) and pcr detection of important virulence genes(MagA and Kfu). Results of examination of 150 chicks samples revealed 16 isolate of Klebsiella with percentage of 10%.gram negative bacilli, non spore forming, capsulated, non motile and arranged singly.on macConkey : lactose fermenting colonies, more or less dome shaped,3-4 mm diameter after overnight incubation at 37C .gave positive reaction for catalase test, voges proskaure test ,citrate test and urease test. Mean while the isolates were negative for indole , oxidase and methyl red tests. Sixteen isolates gave positive result for GyrA gene. Seven Klebsiella Isolates all isolates gave negative results for KPC gene (Klebsiella Pneumoniae Carbapnamase gene) .Ten Klebsiella isolates were tested for detection of Mag A gene using PCR. seven isolates gave positive result for Klebsiella Mag A gene (Mucoviscocity attached gene) responsible for mucoviscocity and attachment to host cells.Ten Klebsiella isolates were tested for detection of Kfu gene using PCR. eight isolates gave positive result for Klebsiella Kfu gene (Iron uptake system gene) this gene is responsible for virulence of Klebsiella. the antibiotics of high effect on isolated Klebsiella were Meropenem, Impienem, Amikacin , Cefotaxime sodium , Ceftazidime and Ciproflaxin . Meanwhile Gentamycin and Cefepime has moderate effect on Klebsiella . Moreover, antibiotics have less effect on the isolated Klebsiella were Azithromycin and Erythromycin.