Abstract
The spillage of oil and other petroleum products have been a major source of environmental pollution globally. Rampant use of chemical remediation methods were shown to be noxious with detrimental consequences. Exploration of microbiodata for probable candidate to be utilized for degradation of oil spillage or contamination is of great interest. In this study, soil samples from 10 different mechanic workshops in mechanic village Dutse were collected as well as control sample from unpolluted hydrocarbon free soil at same site, bacterial species were isolated, identified using biochemical and molecular 16S rRNA gene sequencing and post sequencing analyses. Species enumerated were Bacillus cereaus, Bacillus subtilis, Bacillus licheniformis and Paenibacillus alvei, The sequences were compared to closest relative species in the GenBank data base of National Centre for Biotechnology Information. However, the substrate used was engine oil and all the isolates were found to utilized the test substrate. Biodegradation studies have been carried out through Gas Chromatography Mass Spectrometry (GC-MS). Of all the isolates, Bacillus subtilis might be the most hydrocarbon degrading bacterial species due to high average relative abundance concentration of degraded hydrocarbon compound.
Highlights
The growth of petroleum industry in Nigeria and the demand of petroleum products have made oil pollution a serious environmental problem
The Gas Chromatography Mass Spectrometry (GC-MS) method deployed is as follows: 1μl of the methanolyzed sample mixture consisted of colonies of the test organisms and gel agar around petroleum supplement was injected into the GC-MS at a split ratio of 1:10 and Helium gas was used as a carrier gas at a flow rate of 48 ml/min and 0.40 bar
Five bacterial strains were isolated from ten different oil contaminated garages in mechanic site
Summary
The growth of petroleum industry in Nigeria and the demand of petroleum products have made oil pollution a serious environmental problem. Bone marrow damage and kidney and liver diseases have been associated with the high concentration of petroleum spills compounds [17]. Along this line, natural ecosystems are contaminated with petroleum hydrocarbons, and microbial species are capable of degrading the spilled hydrocarbons [25]. One of the best approaches to restoring contaminated soils is to make use of microorganisms capable of degrading those toxic compounds a process known as bioremediation [9]. Oil contaminated soil samples from mechanic sites in Dutse metropolis were used to isolate petroleum hydrocarbon degrading bacteria. The study is aimed at providing an insight to the utilization of microbial diversity in solving one of the Nigeria’s eminent pollution problem
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