Abstract
Pasteurella multocida type A is the etiologic agent of fowl cholera, a highly contagious and fatal disease of chickens. The present research work was performed for the isolation, identification and molecular detection of P. multocida Type A from chickens. Liver, heart and spleen of suspected dead chicken (n=35) were collected from Gazipur and Pabna districts in Bangladesh. The targeted bacteria from the samples were isolated, identified and characterized based on their morphology, staining, cultural, biochemical characters, pathogenicity test, histopathological study and Polymerase Chain Reaction (PCR). The P. multocida organism was isolated from 11.42% (n=4/35) samples. The organisms were gram negative, non-spore forming rod, non-motile, occurring singly or pairs in Gram staining, whereas in Leishman’s stain, bipolar shaped organisms were observed. All the isolates were found positive for oxidase and catalase tests, produced indole, and fermented glucose, mannitol and sucrose. Necrotic foci in liver and congestion with hemorrhages in heart were found on necropsy. After pathogenicity test, the pathological changes were reconfirmed by histopathology depicting congestion, hemorrhage and lymphocyte infiltration in heart, liver and spleen tissues. In type specific PCR reaction, the organisms were confirmed as P. multocida Type A. In conclusion, P. multocida type A is prevalent among poultry in the studied regions; thus, care must be taken to control of the disease. http://dx.doi.org/10.5455/javar.2015.b104
Highlights
In developing country like Bangladesh, poultry production in rural areas is considered as a primary source of eggs and meat
Polymerase chain reaction (PCR) and agar gel electrophoresis: The primers used for the detection of P. multocida at species and Type level are listed in Table 1 (OIE, 2008)
The prevalence of fowl cholera (FC) (11.42%; n=4/35) in Gazipur and Pabna districts found in this study was lower than the value reported in the earlier studies (Hasan et al, 2010; Hossain et al, 2013; Belal, 2013)
Summary
In developing country like Bangladesh, poultry production in rural areas is considered as a primary source of eggs and meat. Fowl cholera, Histopathology, Pasteurella multocida type A, PCR In Bangladesh, several studies have been reported on isolation and identification of FC based on conventional bacteriological methods (Rahman, 2003; Bag, 2010; Ievy et al, 2013). There is no report on identification of P. multocida type A by molecular approach in Bangladesh.
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