Isolation and Molecular Detection of Klebsiella pneumoniae from Children Affected by Pneumonia in Dinajpur District, Bangladesh

Abstract

The study was conducted to isolate and identify Klebsiella pneumoniae from children affected by pneumonia in Dinajpur district. This research includes sample collection from pneumonia affected children, isolation and identification of Klebsiella pneumoniae from those samples, molecular detection and antibiotic sensitivity of the identified bacteria. In this research, the samples were collected from nasal secretion of children between 6 months to 10 years old. Then isolation, identification and molecular characterization of Klebsiella pneumoniae from those samples were done. The collection of samples and research work was carried out from May, 2020 to April, 2021. All research work was performed in the Bacteriology laboratory of Microbiology Department, HSTU, Dinajpur. To conduct the study, a total 60 samples were collected from 4 different hospitals in Dinajpur district. Then the samples were brought to the Bacteriology laboratory, Department of Microbiology, HSTU and divided into 5 age category. These samples were then processed and cultural tests were performed in various differential and selective media. Then selected samples were chosen for biochemical tests. After analysing biochemical test results, further selected samples were passed for molecular test to identify the presence of Klebsiella pneumoniae. At last antibiotic sensitivity tests were performed.
 In this test, specific primer for Klebsiella pneumoniae was used to detect the presence of bacteria in the samples. After the cultural, biochemical and molecular tests, total 7 (11.66%) samples were positive among 60 isolates. Among those samples, 2 samples were positive between 6 months to 1 year age for the detection of Klebsiella pneumoniae which is around 14.28%, 2 samples were positive between 1 year to 3 years age which is around 15.38%, 1 sample was positive between 3 years to 5 years age which is around 10%, 1 sample was positive between 5 years to 7 years age which is around 8.33% and 1 sample was positive between 7 years to 10 years age which is around 9.09%. Then molecular detection was done by performing PCR test using specific primer for Klebsiella pneumoniae. At last antibiotic sensitivity test was performed which shows that Klebsiella pneumoniae is resistant to Amoxicillin, Ampicillin, Doxycycline, Erythromycin, Penicillin G and sensitive to Gentamicin, Streptomycin, Azithromycin, Levofloxacin, Tetracycline, Neomycin. All the results resemble recent studies as it is a normal inhabitant of human nosocomial pathway but it holds potential threats for children because in immunocompromised condition, infections can be occurred by Klebsiella pneumoniae which can lead to serious illness becoming more and more resistant to antibiotics.