Abstract
"Dust includes many microorganisms such as airborne bacteria. Most types of these bacteria are harmful to humans and causing severe problems. Klebsiella pneumoniae considered as serious nosocomial pathogen that cause pneumonia, UTIs, wound and liver abscesses. One hundred fifty samples isolated randomly from different indoor and outdoor areas during the period November 2014- February 2015. In the indoor isolates bacterium K. pneumoniae was the commonest pathogen (36.70%) followed by S. aureus (22.78%), S. epidermidis (12.65%), Bacillus spp. (10.12%), P. aeruginosa (5.06%), E. cloacae (5.06%), Streptococcus spp.(2.53%), Citrobacter spp.(1.26%) and Pantoea spp.(1.26%). While S. aureus comprises the majority of outdoor bacteria in (41.66%). Using PCR method for detection of Uge gene and followed with direct DNA Sequencing. The multiple sequence alignment (MSA) showed highly conserved area in all studied strains. However, some variants were observed at different positions (24, 124, 152, 163, 165, 185, 199, 200, 207, 209, 248, 279, 284, 291). Also there were changes in a functional whole areas at the studied gene such as (GGCTGG) at the positions (212-217), (ATCCCG) at positions (226-231), (GCCC) at (233-236), (ACCG) at positions (242-245), (GATT) at (263-266), (TCAA) at (269-272). These differences in the gene sequence may indicates special characterizations for the isolated strain."
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
