Abstract
Fifty five Bacillus isolates were isolated from compost, and alkaline silty clay soil (rhizosphere of potato plant) in Shambat, Khartoum North, Sudan, and screened using morphological tests, biochemical and molecular characterization using 16s rDNA analysis., Screening of cellulase producing isolates was done using carboxyl methyl cellulose (CMC) as a substrate at 25°C. Twenty six isolates were found to be cellulase producers. Among the isolates, four isolates, 9+, 23, 20 and 13 showed high potential in producing extracellular cellulase and had an average cellulase activity of 2.89, 3.12, 3.48 and 3.53 Unit/ml, respectively. Genetic distance between the four isolates with high cellulase activity was determined with RAPD analysis based on OPC-3 primer.
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