Abstract
Currently, there is an increase prevalence of antibiotic-resistant bacteria worldwide. Therefore, the need for characterization of naturally occuring antibiotics with less antibiotic resistance is required. Soil resources contains valuable antibiotic producing microorganisms that increasingly being utilized for the production of suitable antibiotics. Therefore, this study aimed at identifying an antibiotic bacteria with ability of producing antibiotic that is isolated from soil samples collected from Al Zarqa provenance, an arid area in Jordan. Morphological and biochemical characterization of the isolates were carried out and found that all of the isolates belong to Bacillus genus. Further confirmation of the characterization of the bacteria was done by ribosomal RNA and PCR. The results reveal that the isolates represent Basilluslicheniformis. These bacilli were further investigated for antimicrobial activities against 6 ATCC human pathogens viz., S. aureus, S. pneumonia, Salmonella typhi., E. coli, P. mirabels and E. cloacae. Additionally, the results of Gas Chromatography Mass Spectrometry (GCMS) of ethyl acetate extracts for B. licheniformis secondary metabolites showed that they contain two main antimicrobial compounds namely Pyrrolo [1, 2-a] pyrazine-1, 4-dione,hexahydro and Trans-13-octadecenoic acid. The present work maybe suggests that soil isolates from the studied arid area include antibiotic producing strains that can be utilized commercially.
Highlights
The field of medicine and pharmacology are currently revolutionized thanks to the invention of antibiotics that successfully that has been fruitful in combating many infectious diseases
This study aimed at identifying an antibiotic bacteria with ability of producing antibiotic that is isolated from soil samples collected from Al Zarqa provenance, an arid area in Jordan
The results reveal that the isolates represent Basilluslicheniformis. These bacilli were further investigated for antimicrobial activities against 6 ATCC human pathogens viz., S. aureus, S. pneumonia, Salmonella typhi., E. coli, P. mirabels and E. cloacae
Summary
The field of medicine and pharmacology are currently revolutionized thanks to the invention of antibiotics that successfully that has been fruitful in combating many infectious diseases. Many infectious microbes have become increasingly resistant to most available antibiotic due to mainly the extensive these antimicrobial medicine[1]. Varied genetic mechanisms, such as mutation, genetic transfer, and epigenetic, have been adopted by resistant pathogens to make them successful adapted to antibiotics[2]. Among the most important species of bacteria that produce medically importan antibiotics are beloging to the genus Bacillus[9]. These species and other of Bacillus are among the most abundant bacterial strains found in soil with the ability of endospore-forming and Gram positive bacteria. This study attempts using biochemical and 16S rRNA to identify the microbial isolates from an arid are, Al Zarqa, of Jordan and to determine their antimicrobial activities
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