Abstract
Isolation and molecular characterization of Acanthamoeba spp. related corneal infections in Iraqi Patients. A total of 96 samples from corneal scraps and contact lenses were gathered from corneal patients for the current trial, which runs from November 2020 to May 2021. All plates were checked, classifying them as positive or negative morphologically identification of Acanthamoeba spp. where PCR analysis, which entails the targeted amplification of nucleic acids, has been carried out, and where The 18s rRNA gene's DF3 region was the focus of the isolated strains' sequences. Three patients had Acanthamoeba spp. infections, according to the findings. then the sequence analysis was performed using the NCBI tool, which showed the presence of 2 different genotypes, two strains were grouped into the T3 genotype, one from T4, then they were registered in GeneBank under number accession (MZ427912, MZ349042) respectively. To our knowledge, these are the first reported cases of Acanthamoeba keratitis in Iraq.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
