Abstract
The use of microbial technologies in agriculture is currently expanding quite rapidly with the identification of new bacterial strains, which are more effective in promoting plant growth. In the present study 18 strains of Pseudomonas were isolated from soil sample of Balochistan coastline. Among isolated Pseudomonas strains four designated as SP19, SP22, PS24, and SP25 exhibited biocontrol activities against phytopathogenic fungi, that is, Rhizopus microsporus, Fusarium oxysporum, Aspergillus niger, Alternaria alternata, and Penicillium digitatum; PS24 identified as Pseudomonas aeruginosa by 16srRNA gene bank accession number EU081518 was selected on the basis of its antifungal activity to explore its potential as plant growth promotion. PS24 showed multiple plant growth promoting attributes such as phosphate solubilization activity, indole acetic acid (IAA), siderophore, and HCN production. In order to determine the basis for antifungal properties, antibiotics were extracted from King B broth of PS24 and analyzed by TLC. Pyrrolnitrin antibiotic was detected in the culture of strain PS24. PS24 exhibited antifungal activities found to be positive for hydrogen cyanide synthase Hcn BC gene. Sequencing of gene of Hcn BC gene of strain PS24 revealed 99% homology with the Pseudomonas aeruginosa strain PA01. The sequence of PS24 had been submitted in gene bank accession number KR605499. Ps. aeruginosa PS24 with its multifunctional biocontrol possessions can be used to bioprotect the crop plants from phytopathogens.
Highlights
Pseudomonas are ubiquitous bacteria which are of industrial significance
Many Antimicrobial compounds, surfactants, and plant growth promoters have been reported by potential plant growth promoting strains colonizing plant roots and bacterial secretions regulate the interaction between plants and PGPR leading to distinctive plant growth promotion special effects [9, 10]
Test Auxin production Siderophore Phosphorous solubilization HCN production Antifungal activity Rhizopus microspores Fusarium oxysporum, Aspergillus niger Alternaria alternata Penicillium digitatum siderophores and inert reaction was observed on triple sugar iron agar slants
Summary
Pseudomonas are ubiquitous bacteria which are of industrial significance. This is because they exhibit various traits in a wide variety of environments. Evidence has highlighted many metabolites produced by Pseudomonas strains such as antibiotics, enzymes, and volatiles and their key role in controlling an array of fungi causing infections in plants has been used for biofertilizer production and formulation [3, 4]. Toxins liberated from plant pathogenic fungi cause budding infectious diseases leading to epidemics and concerns for food safety [5]. There are various epidemics reported because of plant disease caused by microorganisms and have threatened food security in history of mankind [6]. Consortium of P. fluorescens with other plant growth promoting beneficial bacteria strains showed promising plant growth promoting traits [12]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
