Abstract
Objective To establish an optimal method for in vitro culture and amplification of myoblasts from human skeletal muscle. Methods The myoblasts were isolated from human skeletal muscle with two steps combined dissociation, then the harvested myoblasts were purified by the way of different attachment. The cultured cells were identified by morphology, immunohistochemistry and transmission e-lectron microscopy. Growth curves were plotted and analyzed. Results A lot of highly pure myoblasts could be obtained by this procedure and cultured in vitro steadily and rapidly. Conclusion It shows promise for the treatment of DMD, hemophilia, Buergers disease, myocar-dial infarction and chronic heart failure. Key words: Myoblasts; skeletal; Cell separation; Cell culture techniques
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