Isolation and identification of wheat gene TaDIS1 encoding a RING finger domain protein, which negatively regulates drought stress tolerance in transgenic Arabidopsis

Abstract

Drought stress is a major factor that limits the yield and quality in wheat. In this study, we identified an orthologue of the rice gene OsDIS1 (Oryza sativa drought-induced SINA protein 1) in wheat (Triticum aestivum L.) called TaDIS1. TaDIS1 encodes a putative 301 amino acid protein with a C3HC4 RING finger conserved domain at the N-terminal and a SINA domain at the C-terminal. TaDIS1 contains three exons and two introns. qRT-PCR analysis showed that TaDIS1 expression was induced by PEG6000, NaCl, and abscisic acid (ABA) treatment. We generated TaDIS1-overexpressing transgenic Arabidopsis lines. Under drought stress conditions, the transgenic Arabidopsis plants had a lower germination rate, relative water content, and proline contents, with higher water loss, chlorophyll loss, relative electrical conductivity, and malondialdehyde contents compared with the wild type. The antioxidant enzyme (superoxide dismutase, peroxidase, and catalase) activity levels were lower in the transgenic plants. The TaDIS1-overexpressing plants had shorter roots with greater growth inhibition in response to mannitol treatment than the wild type, with increased hypersensitivity to ABA during seed germination and early seedling growth. The expression of stress-related genes in transgenic plants under drought stress suggests that TaDIS1 may function negatively in drought stress by regulating the stress response-related genes.