Abstract
It has been proposed that allotypic trophoblast lymphocyte cross-reactive (TLX) antigens are involved in the maintenance of normal human reproduction. Despite such a potentially important role for TLX antigens, isolation of human TLX proteins has not yet been reported. As an initial step toward elucidation of the structure and function of TLX antigens, we have isolated TLX proteins from Lubrol-solubilized lymphocytes (termed "wTLX") by anti-trophoblast membrane-Sepharose immunoaffinity chromatography. Using Ouchterlony immunodiffusion and immunoelectrophoresis, we have identified an immunoreactive wTLX antigen which forms a single immunoprecipitation line against absorbed anti-trophoblast membrane. From 17.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analyses, a 35-kDa band was determined to be a major protein band in the immunoaffinity-isolated wTLX fraction, along with multiple minor wTLX bands. These results suggest the possible existence of antigenic polymorphism of TLX, with predominant expression of the 35-kDa wTLX antigen in lymphocytes. The strong staining of the TLX antigens with Coomassie Brilliant Blue and Amido Black indicates they are largely proteins. Co-isolation of beta 2-microglobulin in the immunoaffinity-isolated wTLX pool could imply that the wTLX antigens may be unique class I HLA-like antigens. This possibility has yet to be resolved.
Highlights
Ouchterlony immunodiffusionand immunoelectropho- antibodies which recognize and protect thbelastocystic trophresis, we have identified an immunoreactive wTLX oblast during pregnancy [2,3,4]
The placentais a complex endocrine organ which develops nication, we report for the first time the isolation of highly only during pregnancy and is composed of cells of both fetal purified wTLX antigensfrom Lubrol-solubilized human lymand maternal origin
It has been proposed that allotypic TLX antigens are involved in the maintenance of normal pregnancy by modulating maternal rejection of the fetus [2,3,4]
Summary
From the Division of Biomedical Research,Lovelace Medical Foundation, Albuquerque,New Mexico87108. It has been proposed that allotypic trophoblast lymphocyte cross-reactive (TLX) antigens are involved in the maintenance of normal human reproduction. Despite such a potentially important role for TLX antigens, isolation of human TLXproteins has not yet been reported. Theresultantmaternalanti-TLXantibodies would serve as protective blocking antibodies both by promoting allogeneic recognition and by blocking anti-paternal immunoaffinity-isolated wTLX fraction, along with immune responses. Failure toproduce these blocking antibodmultiple minor wTLX bandsT.
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