Isolation and Identification of Three Alkaloids Compounds from Albizia lebbeck L.Leaves and study of Their Antimicrobial Activity Against Pathogenic Bacteria of Urinary Tracts Inflammatory in vitro

Abstract

Three alkaloidic compounds are 3,3-dimethyl-4-(1-aminoethyl)- Azetidin-2-one, 2,4-Bis( hydroxylamino)-5-nitropyrimidine and 2-Amino-4-hydroxy pteridine-6-carboxylic acid were isolated and purified from Albizialebbeck L.leaves. Gas chromatography- mass spectrum technique, thin layer chromatography and columnchromatography were carried out for identification of chemical structures of these compounds. Antimicrobialactivity of the three alkaloids were recorded against pathogenic bacteria of urinary tracts represented byProteus sp., Klebsiella sp., Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. Thehighest inhibition zone diameter was measured for the mixture of alkaloidic compounds againstStaphylococcus aureus bacteria with value equal to 42 mm at concentration 150 mg/ml whereas the highestinhibition zone diameter was recorded for 2-Amino-4-hydroxypteridine-6-carboxylic acid compound againstStaphylococcus aureus bacteria with value equal to 23 mm at the same concentration. Cytotoxicity detectionproved that all alkaloid compounds have no toxicity towards red blood cells. Therefore the three alkaloids canbe used as medicinal herbal substituents to treat urinary tract Inflammatory instead of antibiotics but this workdemands further pharmaceutical and clinical studies.