Abstract
Long-acting thyroid stimulator (LATS) was found in the plasma in each of 7 subjects with circumscribed, pretibial myxedema, which in every instance had developed after surgical or radioiodine treatment of thyrotoxicosis. Using a potent plasma from one of these subjects, a bio-assay method for the quantitative measurement of LATS was developed which was used in subsequent fractionation and recovery experiments. By fractional precipitation of the proteins of LATS plasma by acid potassium phosphate, followed by gel filtration of the active fraction through diethylaminoethyl-Sephadex, LATS was eluted with a single protein moiety and was recovered in 60–80% yield with an 8-fold increase in purity. Analysis by the methods of ultracentrifugation, paper electrophoresis, immunoelectrophoresis, double diffusion in agar, and sucrose gradient separation confirmed the presence of a single protein which was identified as a 7S gamma globulin. Incubation of LATS with antihuman-7S antibody or digestion of LATS by papain d...
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