Isolation and identification of the fungus Alternaria alternata from some wheat fields and the investigation of some of produced the toxins

Abstract

This study was conducted for the purpose of isolating and Identifing the fungus Alternaria alternata isolation from some wheat fields and investigating some of the toxins produced by the fungus A. alternata . The results of some field survey of Najaf province revealed that the incidence of black point disease on the wheat caused by the fungus A. alternata ranged between 13 - 70% .The study showed identification of a new isolate of the fungus A.alternata that has been registered in the National Center for Biotechnology Information (NCBI), under the name: Najaf and under the accession number KX350052.1. The results of the toxicity test using HPLC and GC-MAS showed that the Najaf isolate is able to produce three types of toxins:namely alteratoxin II (ATX II), alternariol (AOH) and alternariol mono methyl ether (AME).