Isolation and Identification of Staphylococcus Spp. and Gram Negative in Meningitis Patients

Abstract

Introduction: Meningitis is known as a disease that Infect the central nervous system (CNS), it is mainly caused by bacteria, mycobacteria, fungi, viruses and parasites that cause disease and death, bacterial infections constitute the highest percentage of the other species, with (56.6%) and viruses (30.2%). Aim: The present study aimed to isolate bacteria cause meningitis and identify these bacteria. Materials and Methods: A total 248 clinical samples of cerebrospinal fluid (CSF) from meningitis patients were collected, for the period of July to October 2018. From the Child Protection Teaching Hospital in the Medical City Complex in Baghdad. All clinical samples were collected and cultured on blood agar, chocholate agar and MacConkey agar or 24 hours. All isolates were identificated depending on macroscopic, microscopic and biochemical tests (Oxidase, Catalase, Gelatinase, Haemolysin, growth coagulase for Staphylococcus spp.) and (Voges-Proskauer, Methyl red, Indol, Citrate, Urase, Kliglar, Catalase, Oxidase, for Gram negative bacteria) and definite with Vitek-2 compact. Results: The results of the study showed growth of 42 samples, 7/16(44%) S. epidermidis, 3/16(19%) S. hominis, 3/16(19%) S.haemolyticus, 2/16(12%) S.aureus, 1/16(6%) S.warneri. Whereas the Gram negative bacteria include, 1(2%) isolate of Pseudomonas putida, Escherichia coli, Klebsiella pneumonia, and 2(4%) isolates Burkholderia cepacia and Acinetobacter baumannii, and 19(45%) isolates were others (Enterococcus faecium, streptococcus thoraltensis, Kocuria kristinae, Leclerciaade carboxylata, Rothiadent ocariosa, Globicatella sulfidifaciens, Pantoea spp., Pantoeaag glomerans). Conclusion: Various species of bacteria appeared but Gram positive Staphylococcus spp. was more predominant.