Isolation and Identification of some Yeasts from some Plants

Abstract

The present study included isolating 71 local isolates of yeasts during three months, starting from 1/10/2021 to 1/1/2022 from different plant sources, as the fruits were obtained from local markets in Mosul, Kirkuk, Erbil and Dohuk and from home gardens. The isolates were diagnosed after culturing on solid nutrient medium MEA (Malt Extract Agar Medium) based on phenotypic tests including colony color, shape, diameter, nature of its edges, height, texture, brightness, surface shape, culture and microscopy at 40X powers to observe the shape of yeast cells and measure their sizes Using (E 10X) ocular and biochemical including Diazonium Blue B (DBB) Color test, growth in 25˚C and 37˚C, assessing ability to utilize nitrate as a sole nitrogen source, assessing preservative resistance of glacial acetic acid, growth at reduced water activities in high carbohydrate levels, growth at reduced water activities and high level of sodium chloride and ability mycelium formation test. The results showed that it belongs to 17 different species of yeasts Debaromyces, Rhodotorula, Pichia, Candida, Kluyveromyces, Geotrichum, Kloeckera, Saccharomyces, Cryptococcus, Zygosaccharomyces and Trichosporon.