Isolation And Identification Of Some Chemical Constituents In Two Different Types Of Fresh Water Macro Algae In Bestansur Village In Suleiman City Kurdistan Region (North Iraq) By Hplc Technique

Abstract

In the present work HPLC analysis for two genus of green algae were collected locally and identified from Bestansur village fresh water in Sulaimani city in north Iraq in October 2011,shows that contain different alkaloid components such as Chara sp. Contain (Kaemfertin 57.0831µgm/ml ,isoquinoline 74.8601µ gm/ml ,corilagin 109.3617µ gm/ml and colocynthitin 57.3929 µ gm/ml) while Spirogyra contain (Gallic acid 77.2055 µ gm/ml ,Kaempfertin 67.8407 µ gm/ml ,isoquinoline 69.6164 µ gm/ml , corilagin 105.8581 µ gm/ml and colocynthitin 85.9098 µ gm/ml). Divert compounds of steroids composition were also analyzed by HPLC technique in two types of macro algae five steroid compounds were detected (stigmasterol 69.9213 µ gm/ml , 73.102 µ gm/ml , campsterol 98.8401 µ gm /ml ,157.8413 µ gm/ml , β-sitosterol 127.8065 µ gm/ml,37.1229 µ gm/ml ,avenasterol 43.15194 µ gm/ml ,72.400 µ gm/ml and clerosterol 89.6958 µ gm/ml ,84.50799 µ gm/ml respectively). In this study HPLC analysis for Chara show that contain( α-tocopherol 334.53417 µ gm/ml , β- tocopherol 175.13087 µ gm/ml and γ-tocopherol 71.7641 µ gm /ml) . In the end of this study ICP analysis for two genus macroalgae shows that contain this element s (Mn,V,Se, Ca,Mg, Cr,As,Pb,Co,Cd and Ni).