Abstract
Clubroot is very detrimental disease to cabbage production so as farmers work on various efforts to control it. The use of fungicides not only ineffective but also pollute the environment, therefore biological control system need to be pursued. The use of antagonistic agents such as Pseudomonas has been widely studied and known effective in suppressing various pathogens. Therefore it is worth trying its effectiveness against Plasmodiophora brassicae, a pathogen of cabbage. The purpose of this study was to obtain indigenous Pseudomonas which effectively suppress the pathogens and may also increase plant growth. Microbes were isolated from the cabbage area using the Kings'B medium with multilevel dilution. All isolates were tested for their effectiveness in pots in a Completely Randomized Design with a concentration of 1.5x106 CFU (Colony Forming Unit) per pot. The variables observed were plant growth, number of club roots, and percentage of disease incidence. Fourteen isolates of Pseudomonas were isolated. Three Pseudomonas isolates were found most effective at suppressing clubroot disease and increasing plant growth. The best isolate obtained was Pseudomonas-6, followed Pseudomonas-9, and Pseudomonas-8.
Highlights
The use of pesticides is oftenCabbage (Brassica olearacea var. ineffective for soil infectious pathogens capitata L.) is a source of vitamins A, B1, C, especially clubroot pathogens and excessive and minerals for the human body, so as the use would highly polluting the environment.vegetable is very important to the people
Three Pseudomonas isolates were found most effective at suppressing clubroot disease and increasing plant growth
Local Bali cabbage production has decreased safety causes limit the use of pesticides
Summary
Cabbage (Brassica olearacea var. ineffective for soil infectious pathogens capitata L.) is a source of vitamins A, B1, C, especially clubroot pathogens and excessive and minerals for the human body, so as the use would highly polluting the environment. TO CONTROL Plasmodiophora brassciae, THE PATHOGEN OF CLUBROOT DISEASE ON CABBAGE I Ketut Suada and Anak Agung Ngurah Gede Suwastika fluorescens strain CHAO, and has been Zhu, 2015) This all informations indicate shown very effective in suppressing various that there is an adaptation problem of an soil infectious diseases. The use of Pseudomonas in seeds or crops temperature and humidity in the new area so can significantly increase the growth and it is necessary to adapt the agents yield of crops in both greenhouse and field physiologically Are the growing environment of temperature The microbes will be used in their habitats so fluctuation, ultraviolet light exposure, pH that there will not need to be much change, water and moisture availability, adaptation at the growth site It is nutrition, and indigenous microflora necessary to look for indigenous microbes in competition Data analysis The data were analyzed according to the Complete Randomized Design (CRD) used and followed by Duncan Multiple Range Test at level of 1% and 5%
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