Isolation and Identification of Pathogenic Bacteria from Urinary Tract Infections in Infants from Different Hospitals of District Peshawar

Abstract

In the present study isolation and identification of pathogenic bacteria, causing urinary tract infection in infants was performed. The research work was carried out at the Microbiology Laboratory, Abasyn University, Peshawar. Samples were collected from different hospitals of District Peshawar. The collected samples were inoculated on different media for identification and isolation such as Nutrient agar, MacConkey agar, Mannitol salt agar and CLED media. For further identification Gram staining and Biochemical tests were performed such as Catalase, Oxidase, Coagulase, Indole and TSI. In total of 90 samples, E. coli was most prominent, which was found to be 52 (57.77%) followed by S. saprophyticus 22 (24.44%), K. pneumonia 11 (12.22%).While least prominent was S. aureus 5 (5.55%). Among the total isolates the percentage of Gram-negative bacteria in UTI patients was higher than Gram-positive bacteria. Whereas the percentage of E. coli was (53%) and K. pneumonia was (14%) while the percentage of S. saprophyticus was (28%) and S. aureus (5%). Among the total 90 isolates, the highest prevalence was observed in female infants about (58.88%) while in male infants, the percentage was (41.11%). Key words: Urinary Tract Infection, Infants, Pathogenic Bacteria.