Isolation and Identification of p-Nitrophenol Degrading Bacteria from Polluted Soil

Abstract

Now-a-days due to an extensive industrialization, urbanization has leads concern towards organic pollution. Among the nitro-aromatic paranitrophenols are widely used as intermediates for the pesticides, dyes manufacturing processes. In the present study, a strain of Pseudomonas aeruginosa was isolated from the polluted soil especially in the vicinity of pesticide, chemical industry. This strain was able to utilize PNP as a sole source of carbon and energy by degrading it. The sub-culturing of these isolate on nutrient agar slants, growth on the Cetrimide agar formed well isolated colonies. The organism also gives bluish, greenish pigmentation on PIA (pseudomonas isolation agar). The organism is found to be gram-negative, exhibiting rods, motile. It is the catalase positive, MR positive, VP negative, Indole production is absent, it could utilize citrate as a sole source of carbon, it could rapidly liquefy gelatin and an oxidase negative. This studies have been showed that isolate NP1 have the ability to degrade the PNP. On the basis of the morphological, cultural and biochemical characteristics it was confirmed that the NP1 isolate have been identified as Pseudomonas aeruginosa.