Abstract
In this study, a scientific method which can be used to improve nitrification process at low temperature in the sewage treatment plant was introduced. The activated sludge samples were taken from aeration tank of the sewage treatment plant when the outside temperature was below 0°C (water temperature below 12 °C). Five kinds of nitrobacteria strains with cold-resistance and higher activity of ammonia degradation were isolated from aeration tanks. The physiological properties showed the five strains were identified into Sphingobacteriaceae、Rhodanobacter sp.、Pseudomonas sp.、Pandoraea sp. and Perlucidibaca piscinae. All of the strains could convert ammonia-nitrogen or NO2- into NO3- in the medium at 10°C. The ammonia and nitrate removal efficiency could be reached 80.9% and 80.3% respectively. Comparing to the unvaccinated one, the removal efficiency can be increased by 50%, which proved the isolated nitrobacteria could be applied to biological nitrification process of sewage treatment at low-temperature.
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