Abstract
The rhizosphere is the area around the root of a plant occupied by a unique population of usefulbacteria known as plant growth promoting rhizobacteria (PGPR). In this study, the isolation andidentification of rhizobacteria from orange (Citrus sinensis) orchards using 16S rRNA gene, as well asbiological and biochemical assays is reported. Analysis of 16S rRNA gene was confirmed bybiological and biochemical assays and showed that the isolated bacteria belong to the genera Bacilli,Enterobacter and Pseudomonas. Accordingly, Enterobacterkobei MR-R2, Pseudomonas putida MRR5,Bacillus cereus MR-R, Bacillus thuringiensis MR-R1 and Bacillus mycoides MR-R4 wereidentified and registered in GenBank. The results of phosphate solubilization testing revealed that themaximum rate of phosphate solubilization was observed for B. thuringiensis, and E. kobei; however,B. cereus, B. mycoides, and P. putida indicated the least activity. It can be concluded that the isolatedstrains (especially B. thuringiensis) are newly identified phosphate solubilizing bacterial strains,among which B. thuringiensis can be used for growth promotion of orange trees.
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