Abstract
315 variants of halotolerant bacteria were isolated from soil samples taken around Starobin potash deposit mined by Belaruskali concern. Nitrogen-fixing isolate CA-6 and phosphate-solubilizing isolate Cp-1 capable to withstand abiotic stress caused by sodium chloride in concentration 1710 and 2565 mM, respectively and water deficiency characterized by osmotic potential -0.20 and -0.42 MPa were sorted out. Isolate Cp-1 may dissolve phosphates yielding halo zones 9 mm in diameter while isolate CA-6 displays nitrogenase activity 44.0 nM C2H2/vial/3 days. Both isolates are growth-promoting organisms, distinguished by the ability to synthesize IAA in concentrations 89.7 and 37.8 μg IAA/ml of cultural liquid, respectively. The selected salt-resistant isolates were identified based on analysis of the nucleotide sequence of the 16S rRNA gene and data of MALDI-TOF mass spectrometry and were referred to the species Rhodococcus jostii and Priestia megaterium. Halotolerant strains Priestia megaterium Cp-1 and Rhodococcus jostii CA-6 were deposited in Belarusian collection of non-pathogenic microorganisms under the respective registration numbers BIM B-1314D and BIM B-1353D.
Highlights
315 variants of halotolerant bacteria were isolated from soil samples taken around Starobin potash deposit mined by Belaruskali concern
Submerged culture of isolates Ср-1 (a), СA-6 (b), ФП2/1 (c) subjected to osmotic stress induced by various levels of sodium chloride: 1 – LB medium + 1197 мМ NaCl; 2 – LB medium + 1710 мМ NaCl; 3 – LB medium + 2052 мМ NaCl
Впервые выделены и идентифицированы азотфиксирующий штамм Rhodo coccus jostii СА-6 и фосфатсолюбилизирующий штамм Priestia megaterium Ср-1, способные переносить абиотический стресс, вызванный хлоридом натрия в концентрациях 1710 и 2565 мМ соответственно и дефицитом влаги при водном потенциале ‒0,20 и ‒0,42 МПа
Summary
Скрининг азотфиксирующих изолятов проводили среди отобранных 43 солеустойчивых бактерий, которые росли на среде, содержавшей хлорид натрия в концентрации 1710–2565 мМ, путем посева на твердые элективные питательные безазотистые среды Эшби и Бёрка Из выделенных и отобранных 43 солеустойчивых изолятов 28 имели хороший или средний рост на безазотистых средах Эшби и Бёрка. 1. Электрофореграмма ПЦР-анализа солеустойчивых изолятов бактерий с парой праймеров nifH-1F и nifH-1R: 1 – Е10; 2 – E. coli (отрицательный контроль); 3 – DST72; 4 – DST73; 5 – DST74; 6 – DST75; М – маркер молекулярной массы ДНК (100 bp); 7 – DST76; 8 – DST77; 9 – DST78; – DST101; – DST102; – DST103; – DST104; – ФП2/1. Effect of halotolerant isolates on seed germination, growth and development of small radish seedlings
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