Isolation and Identification of Major Pathogenic Bacteria from Clinical Mastitic Cows in Asella Town, Ethiopia.

Gezehagn Kasa,Betelihem Tegegne,Belege Tadesse,Sumanta Nandi

doi:10.1155/2020/6656755

Abstract

Mastitis is a multietiological and complex disease causing inflammation of the parenchyma of mammary glands and is a problem in many dairy cows. The objective of this study was to isolate and identify the pathogenic bacteria that cause bovine clinical mastitis. A cross-sectional study was undertaken between November 2018 to April 2019 on a small scale and government dairy farms in Asella town. Cow's udder and teats were physically examined to detect clinical mastitis. A total of 83 milk samples were collected from 46 cows that show clinical sign of mastitis from a total of 12 farms. Isolation and identification of major bacterial species were carried out by culturing different media and using primary and secondary biochemical tests. Out of the 83 samples collected and examined, all (100%) were positive for the cultural isolation of bacterial species. The bacteria were identified to genus and species level. Among the 83 isolates, 32 (38.6%), 24 (28.9%), and 6 (7.2%) were Staphylococcus aureus, Staphylococcus intermedius, and Staphylococcus hyicus, respectively. Other bacteria like Escherichia coli 12 (14.5%) and Streptococcus species 2 (2.4%) were also isolated. Bacillus species 2 (2.4%), Proteus species 2 (2.4%), and 3 (3.6%) of them were mixed bacterial infections. The present study revealed that both contagious and environmental bacterial pathogens were responsible for the occurrence of clinical mastitis. Proper milking practices and farm husbandry practices and future detailed studies up to the species level and on antibiotic profiles of the pathogens are needed.

Highlights

Ethiopia is believed to have the largest livestock population in Africa [1]. e total cattle population in the country is estimated to be about 56.71 million
All (100%) the 83 milk samples collected from clinically mastitic cow were positive for the cultural isolation of bacterial species. e bacteria were identified to genus and species level
A total of 83 milk samples were collected and processed from clinically infected cows from small scale holder and government dairy cows in Asella town. e result of the current study showed that Staphylococcus species, Streptococcus species, Escherichia coli, Bacillus species, and Proteus species were isolated which has been reported in another study [20]

Summary

Introduction

Ethiopia is believed to have the largest livestock population in Africa [1]. e total cattle population in the country is estimated to be about 56.71 million. Ethiopia is believed to have the largest livestock population in Africa [1]. E total cattle population in the country is estimated to be about 56.71 million. From the total cattle 98.66% in the country are local breeds and the remaining are hybrid and pure exotic breeds that accounted for about 1.19 and 0.14%, respectively [1]. Mastitis is among the various factors contributing to reduced milk production. Bovine mastitis is the second most frequent disease next to reproductive disorders and one of the major causes of economy failure in Ethiopia. It affects both the quantity and quality of milk [3]

Methods

Results

Discussion

Conclusion