Isolation and identification of low-density polyethylene (LDPE) biodegrading bacteria from waste landfill in Yazd

Abstract

ABSTRACTMicroorganisms used as a biodegradation technique can provide economic benefits and can be compatible with the environment. The aim of this study was to isolate low-density polyethylene (LDPE) degrading bacteria. The waste samples were collected from Yazd landfills. Biochemical and molecular tests based on 16S rDNA sequencing were done to identify the superior isolates. Biodegradation rate was measured using weight loss measurement, spectroscopic analysis with Fourier-transform infrared (FTIR) spectroscopy and an investigation of surface morphology changes using scanning electron microscopy (SEM). Two biodegrading isolates were identified as Pseudomonas aeruginosa strain SKN1 and strain SKN2. The weight loss of LDPE strips was measured at 10.32%. SEM micrographs showed the surface degradation and colony formation on LDPE strips. The FTIR spectrum revealed the structural changes on LDPE strip surfaces. Bio-decomposition was observed to have taken place and the bacterial strains had a special ability to biodegrade LDPE wastes.