Abstract
The red pakoba (Syzygium sp.) is a plant originating from north sulawesi that is often used by local people as a basic ingridient of medicine. This research was conducted to isolate and identify glycoside compounds contained in the n-butanol fraction of red pakoba fruits (Syzygium sp.). The isolation process was carried out using the maceration method, for the separation and purification of compounds using Thin Layer Chromatography (TLC) and Gravity Column Chromatography and obtained F2.4 sub-fraction isolates with a sample weight of 4.5 mg. Identification of compound using Proton Nuclear Magnetic Resonance (NMR-1H) and Carbon Nuclear Magnetic Resonance (NMR-13C) tecniques. The result of identification of isolate F2.4 sub-fraction as flavonoid glycoside compounds with the proposed name 3-O-methyl-butyrate-6,7,3',4' tetrahydroxy-5-methoxy-8-methyl flavan with molecular formula (C22H25O8)
Highlights
Red Pakoba an endemic plant from NorthSulawesi Province, this plant belongs to the genus Syzygium which has similar properties to other plants that have similar Latin names such as Syzygium aromaticum, Syzygium Cumini (L), and Syzygium luzonense
The fruit of this plant is used by the local people as a basic ingredient for making drugs because it has pharmacological functions such as antioxidants and antidiabetic [1,2,3,4,5,6,7,8,9,10]
Flavonoid compounds are compounds that are commonly found in plants, no exception to Red Pakoba (Syzygium sp.)[14]
Summary
Sulawesi Province, this plant belongs to the genus Syzygium which has similar properties to other plants that have similar Latin names such as Syzygium aromaticum, Syzygium Cumini (L), and Syzygium luzonense. Material and Method Plant sample the Red Pakoba fruit (Syzygium sp.) obtained from Laikit Village, located in North Minahasa Regency, size 230400 mesh silica gel, silica gel 60 GF254, butanol, ethyl acetate, dichloromethane, methanol, distilled water, Thin Layer Chromatography (TLC), 10% H2SO4 solution in ethanol as stain emerger, and other materials such as Whatman 42 filter paper, aluminium foil, and label paper. Extraction stage Red pakoba fruit (Syzygium sp.) was mashed using a blender, macerated 7x24 h with ethanol solvent whose level is 70%, allowed to stand for 24 hours until the filtrate is obtained. Eluate which still has two or more stains on TLC, will have to be going under column chromatography again This stage is continued until a pure compound is obtained. The pure compound was further analyzed using the Proton Nuclear Magnetic Resonance (H-NMR) and Carbon Nuclear Magnetic Resonance (C-NMR) spectrometry
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