Isolation and Identification of Fungi from Different Types of Vegetables in Erbil Local Markets

Abstract

A survey study was undertaken for the isolation and identification of vegetables-borne fungi at different areas in local markets in Erbil city. Fungi were isolated from vegetables during this study's period by directly method and indirectly method. Directly method by taking a part of the tissue a round infected zone by 1cm, the samples is transfer to a Petri dish or beaker then cuts into small cube pieces, after washing it by SDW, and then the pieces transfer to a Petri dish contains PDA, and indirectly the samples is submerged in sodium hypochlorite 6% for 1-2 minutes, then washed by SDW three times each for 1-2 minutes, then the pieces transfer to a Petri dish contains PDA. Seven different genera of fungi were isolated in both methods. The most frequently isolated fungi were Rhizopus spp. and Fusarium spp. While the least frequently isolated fungal genera were Rhizoctonia sp. and yeasts. The study of antagonistic phenomenon show that the antagonistic genus ( Trichoderma harzianum ) was significantly inhibited the radial growth of the vegetables borne fungi tested, ( Rhizopus sp., Fusarium sp. and Aspergillus sp.) with different ratios.