Isolation and Identification of Ferulic Acid From Aerial Parts of Kelussia odoratissima Mozaff

Seyed Ebrahim Sajjadi,Narjess-Sadat Moayedi,Yalda Shokoohinia

doi:10.5812/jjnpp.4861

Abstract

Background: Kelussia odoratissima Mozaff. is one of the newest genera of Umbelliferae which is represented by only one species. This sweet-smelling, self-growing monotypic medicinal plant is endemic to a restricted area in west of Iran and is locally called Karafse-koohi. The aerial parts of the plant are commonly used as a popular garnish and a sedative medicinal plant. There are several reports concerning antioxidant, anti-inflammatory, anxiolytic and hypolipidemic activities of aerial parts of K. odoratissima. Objectives: The current research aimed to evaluate some phenolic contents of the plant for the first time .It is confirmed that secondary metabolites and especially phenolic compounds have important role in the biological activities of the plant. Available information indicates that phenolic contents of K. odoratissima have not been the subject of any investigation. Material and Methods: Aerial parts of K. odoratissima were extracted with acetone by maceration method. Normal and reversed phase vacuum liquid chromatography used to fractionate the extract. 1H-NMR, 13CNMR, EI-Mass and IR spectra were used to elucidate isolated compound. Results: The phenolic acid isolated compound was identified as 4-hydroxy-3-methoxycinnamic acid (ferulic acid). Conclusions: Compared with previous reported antioxidant and anti-inflammatory properties of ferulic acid, a chemical-biological relation can be postulated.

Highlights

Kelussia odoratissima Mozaff. is one of the newest genera of Umbelliferae which is represented by only one species
The 13CNMR spectrum showed the presence of 10 signals (6 aromatic carbon and 4 aliphatic chain) in agreement with the proposed structure of ferulic acid (4-hydroxy3-methoxycinnamic acid) (Figure 1)
Ferulic acid (4-hydroxy-3-methoxycinnamic acid), is a phenolic acid compound which arises from the metabolism of phenylalanine by shikimic acid pathway in plants

Summary

Introduction

Kelussia odoratissima Mozaff. is one of the newest genera of Umbelliferae which is represented by only one species. Is one of the newest genera of Umbelliferae which is represented by only one species This sweet-smelling, self-growing monotypic medicinal plant is endemic to a restricted area in west of Iran and is locally called Karafse-koohi. Kelussia is one of the newest genera of this family and is represented by only one species, Kelussia odoratissima Mozaff., which is found only in Iran [2] This sweet-smelling, self-growing monotypic medicinal plant is endemic to a restricted area in western Iran and is locally called “Karafse-koohi”. The antioxidant activity of the methanolic extract of Isolation and Identification of Ferulic Acid the plant was evaluated by β-carotene bleaching assay, reducing power, thiocyanate, accelerated oxidation of sunflower oil, and DPPH radical-scavenging which was effective in some assays [3, 4]. Essential oil of the plant showed a pronounced antibacterial effect [9] and folkloric proposed use of the plant as anti-inflammatory agent was confirmed via a carrageenan test [10]

Objectives

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Conclusion