Isolation and Identification of Endophytic Fungi from Leaves, Stem and Flower of Clerodendrum thomsoniae Balf. f.

Abstract

Abstract: Endophytic fungi are those living inside the host plant without causing any apparent negative effect on the host plant. 117 isolates of endophytic fungi were isolated from the leaves, stem and flower of Clerodendrum thomsoniae. Collected from different areas of Mysore. All isolates were identified based on characteristics of the isolates such as colony appearance, mycelial texture, conidial spores size, shape, pigmentation, and other morphological characteristics observed using a microscope. The isolates of endophytic fungi were identified are Ascomycota and Basidiomycota and classified genera Sterile hyphae, Lasiodiplodiae sp, Rhizopus stolonifera sp, Aspergillus sp, Aspergillus conidiophore, Mycelium of Lentinus, Nigrospora sp, Nigrospora zimmermaniae sp, Curvularia sp, Collectrotrichum sp, Aspergillus fumigates sp. The overall colonization rate of endophytes in leaves, stem and flower were found to be 96.66%. The leaf showed a low percentage of colonization frequency of the endophytic fungi when compared to stems and flower segments.