Isolation and Identification of Endophytic Fungi Associated with Indonesian Sesbania grandiflora Plant

Abstract

In the present study, endophytic fungi associated with Indonesian Sesbania grandiflora plant were isolated and identified for the first time. The objective of this study was to report new data regarding the endophytic fungi found in S. grandiflora as one of Indonesian medicinal plant. Six isolates of endophytic fungi were isolated from the leaves, bark, seed, flower, and root of S. grandiflora collected from Labuhan Ratu, Kedaton, Bandar Lampung, Indonesia. Based on the prediction of their morphological characteristics visually, four isolates from all parts of plant were identified as Fusarium sp, while two isolates obtained from the bark and the seed were identified as Hormiscium sp and Penicillium sp, respectively. In addition, TLC profile result of secondary metabolites extract of endophytic fungi indicated that more than one major compound was observed. Furthermore, the antibacterial screening of isolated endophytic fungi did not show inhibition growth against resistant E. coli. However, the phytopharmacological study on the isolated fungi associated with S. grandiflora as well as their biological properties are still in progress. The results of this study revealed that S. grandiflora plant is reliable source of endophytic fungi for the future investigation.