Isolation and Identification of Defense Responsive Genes in Wheat During Incompatible Interaction withBipolaris sorokiniana(Cochliobolus sativus) using SSH Technique

Abstract

During the initial phase of host pathogen interaction, induction of defense genes is very critical for the incompatible interactions. In order to isolate the defense responsive genes during interaction of wheat with spot blotch fungus, Bipolaris sorokiniana, suppressive subtraction hybridization (SSH) technique was used. Four hundred and eighty clones were obtained from cDNA subtraction library and out of these 36% (172) were of high quality which were sequence analysed using n BLAST from NCBI database. Forty nine differentially expressed clones were obtained which were classified into seven classes based on their putative functions. Twelve clones were classified as plant defense responsive genes based on their putative functions observed in NCBI database. Clone SSH_Wh 135 matched with putative puroindoline b proteins from Triticum aestivum which are encoded by puroindoline gene (pin B gene). This gene contains tryptophan rich hydrophobic domain, which is responsible for grain hardness and also has antimicrobial properties having lipid binding sites.