Isolation and identification of Circovirus in pigeon

Safwan Al-Baroodi,Mozahim Al-Attar

doi:10.33899/ijvs.2020.126706.1364

Abstract

The purpose of this study is first trial to detect of pigeon circovirus, so 1sr group include 100 cloacal swabs were collected 55 healthy and 45 ill pigeons, 36 yearlings and 64 adults, the 2nd group included organs was liver, spleen, bursa of Fabricius from 41 young pigeons 10-30 days old and bursa of Fabricius, liver, spleen from 28 dead in shell pigeon embryo in the 3rd group. DNA extracted from this samples and detection of virus DNA was attempt using polymerase chain reaction, after DNA amplification, the final products of the amplicon with 331 bp was cleared by using electrophoresis using agarose gel at concentration 2%. Results of viral DNA amplification were positive, which revealed as band in 331 bp the results showed that ill yearling pigeons recording high infectivity rate 66.7% compare with healthy yearling pigeons and adult once, the bursa of Fabricius samples of dead yearling pigeons recorded high prevalence 36.58% when compare with liver and spleen samples, DNA of pigeon circovirus high detected 60.71% in bursa of Fabricius of dead in shell pigeon embryo.in conclusion pigeon circovirus affected the racing pigeon in Mosul, Iraq.

Highlights

Pigeon circovirus belongs to the genus Circovirus, family Circoviridae
DNA extracted from this samples and detection of virus DNA was attempt using polymerase chain reaction, after DNA amplification, the final products of the amplicon with 331 bp was cleared by using electrophoresis using agarose gel at concentration 2%
Results of viral DNA amplification were positive, which revealed as band in 331 bp the results showed that ill yearling pigeons recording high infectivity rate 66.7% compare with healthy yearling pigeons and adult once, the bursa of Fabricius samples of dead yearling pigeons recorded high prevalence 36.58% when compare with liver and spleen samples, DNA of pigeon circovirus high detected 60.71% in bursa of Fabricius of dead in shell pigeon embryo.in conclusion pigeon circovirus affected the racing pigeon in Mosul, Iraq

Summary

Introduction

Pigeon circovirus belongs to the genus Circovirus, family Circoviridae. It classified with other viruses in same genus including duck circovirus, porcine circovirus 1 and 2, canary circovirus, goose circovirus. The virus has wide range hosts including several domestic and wild birds causing variety of clinical signs including loss of weight, loss of feathers, dullness, and the crop impacted with yellowish fluid, poor racing, poor growth, abnormal in respiration, severe diarrhea, immunosuppression which cause activation of other secondary infection and the mortality rate reach up to 20% [4]. These clinical signs are merged in a term Young Pigeon Sickness (YPS) [5,6]. There are no vaccines against pigeon circovirus but recently the researchers can be developed subunit vaccine (capsid) but this vaccine is under experimental condition only [10]

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Results

Conclusion