Isolation and identification of chemical constituents from the roots of Peucedanum praeruptorum

Abstract

The dried roots of Peucedanum praeruptorum Dunn, Baihuaqianhu, is well known traditional Chinese medicine. It has been used as anti-asthma, antitussive and as a remedy for angina. Recent pharmacological studies showed that the extract of P. praeruptorum has various beneficial pharmacological activities. Chromatographic separation of a 70% EtOH extract of roots of P. praeruptorum led to the isolation of a new angular-type pyranocoumarin, along with fifteen known compounds including twelve pyranocoumarins, two furanocoumarins, and a polyacetylene. The structures of all compounds were determined on the basis of 1D and 2D NMR techniques (COSY, NOESY, HSQC, HMBC) in association with HR-ESI-MS data analysis. Their stereochemistry were determined by optical rotation and circular dichroism studies. All of the isolates were evaluated for their protective activity against chemotherapy-induced myelosuppression using ex vivo hematopoietic CFC assay system. Four compounds isolated from P. praeruptorum showed protective effects. In this study, we report the isolation and structural elucidation, and biological activities of these compounds from the roots of P. praeruptorum.