Isolation and Identification of Benzochroman and Acylglycerols from Massa Medicata Fermentata and Their Inhibitory Effects on LPS-Stimulated Cytokine Production in Bone Marrow-Derived Dendritic Cells.

Ya Sun,Wei Li,Young Kim,Young-Sang Koh,Seo Yang

doi:10.3390/molecules23092400

Abstract

Massa Medicata Fermentata (MMF), known as Shenqu, is an important traditional Chinese medicine widely used to treat indigestion, vomiting, and diarrhea. In this study, a new benzochroman, 3(S)-3,4-dihydro-5,10-di-β-d-glucopyranoside-2,2-dimethyl-2H-naphtho(2,3-b)pyran-3-ol (1), and five known galactosyl acylglycerols (2–6) were isolated from a methanol extract from MMF. In addition, their chemical structures were determined by chemical and spectroscopic methods, which were compared with the previously reported data. Furthermore, the effects of isolated compounds on lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells were investigated. Compounds 1–3 exhibited significant inhibitory effects on the LPS-induced production of IL-6 and IL-12 p40, with IC50 values ranging from 1.6 to 10.2 μM. Compounds 2 and 3 also exhibited strong inhibitory effects on the LPS-stimulated production of TNF-α with IC50 values of 12.0 and 11.2 μM, respectively. The results might provide a scientific basis for the development of the active components in MMF, as well as for novel anti-inflammatory agents.

Highlights

Massa Medicata Fermentata (MMF), known as Shenqu, is one of the widely used traditional medicines [1]
MMF has been widely used for treating indigestion, vomiting, diarrhea, obesity, and related metabolic diseases
The present phytochemical and pharmacological study on MMF searched for anti-inflammatory constituents and unique compounds, which resulted in the isolation of a new benzochroman derivative (1) from an MMF methanol extract

Summary

Introduction

Massa Medicata Fermentata (MMF), known as Shenqu, is one of the widely used traditional medicines [1]. MMF is fermented using six traditional medicinal materials: wheat L.); red bean (Vigna umbellata (Thunb.)); bitter almond (Prunus armeniaca L.); sweet wormwood (Artemisia annua L.); cocklebur (Xanthium sibiricum L.); and water pepper MMF has been widely used for treating indigestion, vomiting, diarrhea, obesity, and related metabolic diseases. Recent pharmacological research showed that MMF demonstrates significant anti-inflammatory activity in an LPS-mediated inflammatory insult, either individually in vitro using. The active components have not been reported. The present phytochemical and pharmacological study on MMF searched for anti-inflammatory constituents and unique compounds, which resulted in the isolation of a new benzochroman derivative (1) from an MMF methanol extract

Methods

Discussion

Conclusion