Isolation and identification of bacterial flora from respiratory tract of healthy horses

Mt Rahman,Sml Kabir,Zb Muktha

doi:10.3329/jbau.v13i2.28785

Abstract

This study was carried out during the period of July to December, 2014 in order to isolate and characterize bacterial flora present in the respiratory tract of healthy horses in and around BAU campus. Eighteen apparently healthy horses were used for the study. Swab samples were collected from the nasal cavity. The bacteria was isolated ,identified and characterized by cultural (aerobic condition),staining, biochemical and PCR technique. Each of the samples collected yielded at least one bacterial species. A total of 27 bacteria were isolated from the selected animals. The majority of the isolates (15/27, 55.56%) were Gram-positive and the rest (12/27, 44.44%) were Gram-negative. Bacterial isolates were Staphylococcus aureus (83.33%) and E. coli (66.66%).The antimicrobial susceptibility pattern of bacterial isolates was investigated by disc diffusion method. The antibiotic sensitivity test of Staphylococcus aureus revealed that the isolates were highly sensitive to ciprofloxacin, moderately sensitive to gentamicin and resistance to amoxicilin, ampicilin and erythromycin. On the other hand, E. coli showed moderately sensitive to ciprofloxacin and gentamycin, mildly sensitive to erythromycin and resistance to amoxicilin and ampicilin. Detection of E. coli and S. aureus from the respiratory tract of healthy horses were not unexpected. Ciprofloxacin and gentamicin could be used for therapeutic purpose, if diseases occur by these organisms in horses.J. Bangladesh Agril. Univ. 13(2): 239-246, December 2015

Highlights

The common history of humans and horses date at about 3500 B.C
It is a matter of hope that, with the pace of modern world the people of Bangladesh are being more conscious about horse
It's worth noting that every exposure to the allergen causes inflammation that lasts for days, so horses that are outside during the day and only exposed to dust and allergens in the stable at night or in the arena are still at risk (Davis et al, 2007)

Summary

Introduction

The common history of humans and horses date at about 3500 B.C. The archeological vestiges show that horses were used in welfare. Exercising in frigid temperatures has been shown to cause inflammation in the lungs and Isolation and identification of bacterial flora of healthy horses airways, and may be a large factor in the development of respiratory infections and asthma (Davis et al, 2005). Considering the above mentioned scenarios present study was carried out with the objectives (a) To determine the prevalence of bacterial flora found in respiratory tract of healthy horse based on their isolation and identification and (b) To determine the antibiogram of the isolated bacterial flora.

Results

Conclusion