Isolation and Identification of Bacteria Present on Spectacles

Abstract

The human eyes have a special anatomy that makes them vulnerable to infectious agents, including bacteria, as well as direct environmental exposure. The eyelids, conjunctiva, and cornea are the main parts of the eyes that are most frequently infected. The numerous microorganisms have been linked to eye infections caused by microbial contamination and colonization of eyeglasses. A study reported the presence of bacterial contaminants on spectacles used by students at Shri Shivaji Science and Arts College Chikhli, in July 2023 to August 2023, Such as Pseudomonas sp., Staphylococcus aureus, Staphylococcus epidermidis, and Bacillus sp. Total 40 samples are collect from different students such as 14 samples are collected from 11th Students, 12 samples are collected from 12th Students, 08 samples are collected from B.Sc. Students and 06 Samples collected from M.Sc. Students were taken by using sterile cotton swabs that had been moistened with sterile distilled water. After that it was inoculated on the Blood agar and MacConkey agar by streak plate method and incubated for 24 hours at 37°C. After incubation for 24 hours at 37°C, for confirmed the growing microorganism by using different tests such as Gram Staining, Hanging-Drop method, IMViC test, Carbohydrate fermentation, Enzyme production (Catalase Test and Oxidase Test), and Triple Sugar Iron Agar test are performed as per required for confirmation. All samples shows positive results, when we examined results, it shows different bacteria are present such as Bacillus sp. (55%), Pseudomonas sp. (42.50%), Staphylococcus aureus (22.50%), and Staphylococcus epidermidis (12.50%). The results shows that the there was contamination by pathogenic bacteria on spectacles used by the students of Shri Shivaji Science and Arts College Chikhli. It means spectacles provide suitable condition for survive of pathogenic bacteria then its leads to the cause of eyes infection.