Abstract
Aim:The study was conducted for the isolation, identification, and antibiogram of bacteria obtained from fresh guava (Psidium guajava).Materials and Methods:A total of 25 fresh guavas were collected from five markets located in Mymensingh city. Guava samples were cultured onto various selective media such as eosin methylene blue, xylose lysine deoxycholate, thiosulfate-citrate-bile salts-sucrose, blood agar, and mannitol salt agar for the isolation of bacteria. Biochemical tests (dextrose, maltose, lactose, sucrose, mannitol, methyl red, Voges–Proskauer, and indole) were performed to identify the bacteria.Results:Total viable counts of guava were ranged between log 6.56 colony-forming unit (cfu)/ml and 6.62 cfu/ml. A total of 106 bacterial isolates belonged to five genera (Escherichia coli, Salmonella spp., Vibrio spp., Bacillus spp., and Staphylococcus spp.) were identified. Salmonella spp. (23.6%) was the most prevalent, followed by E. coli (22.64%), Bacillus spp. (19.81%), Staphylococcus spp. (17.92%), and Vibrio spp. (16.03%). The results of antibiotic sensitivity test showed that Salmonella spp., Bacillus spp., and E. coli were sensitive to chloramphenicol, ciprofloxacin, and gentamicin and resistant to ampicillin and cephalexin. Vibrio spp. was sensitive to chloramphenicol and gentamicin, intermediately sensitive to ciprofloxacin and ampicillin and resistant to cephalexin.Conclusion:The results of this study indicate that fresh guava contains multidrug-resistant bacteria which might pose a public health risk.
Highlights
Among indigenous fruits, guava is one of the popular fruits in Bangladesh
A total of 106 bacterial isolates belonged to five genera (Escherichia coli, Salmonella spp., Vibrio spp., Bacillus spp., and Staphylococcus spp.) were identified
The results of this study indicate that fresh guava contains multidrug-resistant bacteria which might pose a public health risk
Summary
Guava is one of the popular fruits in Bangladesh. Guava is often called the “apple of the tropics” belonging to the family Myrtaceae and the scientific name is Psidium guajava. Guava stands fifth in production among the most important fruit crops of Bangladesh and is grown all over the country. According to “Yearbook of Agricultural Statistics-2015,” annual guava production in Bangladesh during 2014-2015 from inside and outside garden is 2,06,425 metric tons [1]. Pharmacological, digestive, and nutritive values with pleasant flavor, high palatability, and availability in abundance at a moderate price [2]. Guava is a fresh fruit which is consumed raw without processing, posing a potential food safety problem by any pathogenic microorganism
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
