Isolation and identification of associated bacteria and maggots from myiasis affected wounds of cattle and goats in Bangladesh

Md Islam,Md Rahman,Abdullah Maruf,Md Samad,Muhammad Hossain,Md Tarafder,Hossain Rahman,Abdullah Noman,Md Mannan

doi:10.5455/javar.2015.b55

Abstract

The study was carried out to investigate the associated maggots and bacteria in myiasis affected wound in animals. Total 37 myiasis affected cattle (n=25) and goats (n=12) were examined in this study, of which 21 samples were collected from the Veterinary clinics of Bangladesh Agricultural University (BAU), Mymensingh, and the remaining 16 samples were collected from Upazila Veterinary Hospital, Gouripur, Mymensingh. The maggots were collected from the wounds, and were identified under microscope. For microbial identification, samples (n=10) were collected aseptically by using sterile cotton swab. The samples were allowed to grow in media. Colony and staining characteristics were studied. Traumatic, creeping or migratory myiasis wounds were identified in the examined animals. In this study, subcutaneous wound was mostly recorded, of which 43% (n=9/21) was found at the Veterinary Clinics, BAU, and 44% (n=7/16) was found at the Upazila Veterinary Hospital, Gouripur. The maggots were identified as Chrysomya bezziana, commonly known as Old World screwworm. Occurrence of myiasis due to C. bezziana larvae was recorded as 100%. The associated bacteria were identified as Staphylococcus spp. In conclusion, the myiasis wounds are associated with both Staphylococcus spp. and larvae of C. bezziana. Preventive and control measures against the C. bezziana and Staphylococcus spp. can be undertaken to save the livestock from myiasis in Bangladesh.

Highlights

Myiasis is an invasive infestation in the body of vertebrates caused by maggots of flies
Experimental animals: The research was conducted with the patients that were represented to two Veterinary Hospitals namely Veterinary Clinics of Bangladesh Agricultural University (BAU) and Upazila Veterinary Hospital, Gouripur, Mymensingh
The findings of the present study of myiasis wound were closely agreed with the findings of Mcgraw and Turiansky (2008) who reported that cutaneous involvement was the most common type of myiasis

Summary

INTRODUCTION

Myiasis is an invasive infestation in the body of vertebrates caused by maggots (larvae) of flies. Myiasis is a well-known condition to veterinarians as this condition in animals are frequently found especially in underdeveloped regions. It causes severe economic losses worldwide (Otranto, 2001). Infestation by fly larvae is much more prevalent in animals, it is frequently found in humans especially in rural, tropical and subtropical regions. There are reports on wound myiasis of animals, but few such work on integrated assessment of severity of wound caused by maggots are available. The present research work was undertaken to investigate the wound types found in myiasis, identification and characterization of maggots causing myiasis in cattle and goat, and identification of bacteria infecting the myiasis wound

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION