Isolation and Identification of antibacterial compound producing Streptomyces parvulus ICN698 from a Wetland Ecosystem of Kanyakumari

Abstract

Objective: The alarming threats of drug resistant pathogens have created the search of new antibiotics from different environmental niches. The goal of this study was to identify the antibacterial compound producing actinomycete from Therur pond wetland ecosystem and to isolate the active principle by bioassay-directed isolation. Methods: Actinomycete strain ICN698 was isolated and screened through double layer agar against drug resistant bacterial pathogens. Identification of the antagonistic species was carried out through 16S rDNA sequencing analysis. Production, extraction and TLC bioautography based partial purification was carried out and the Minimal inhibitory concentration value was determined using broth dilution method. Results: The antibacterial active strain was identified as Streptomyces parvulus ICN698. TLC bioautography revealed the Rf value of the active principle were detected at 0.11 and 0.29. Minimal inhibitory concentration of the partially purified compound showed 16 ?g/ml against MRSA, K. pneumonia, A. baumannii and Enterobacter sp. and 64 ?g/ml of MIC against P. aeruginosa. Conclusion: The antibacterial active strain ICN698 from Kanyakumari wetland ecosystem proves to be a good source of antimicrobial agent for further studies. Key Words: Wetland ecosystem, TLC bioautography, 16S rDNA sequencing, Enterobacter sp., Streptomyces parvulus