Abstract

Acanthopanax sessiliflorus (Rupr. & Maxim.) Seem. (Araliaceae) is one of the most abundant species of genus Acanthopanax. The fruits of A. sessiliflorus are used in traditional medical protocols as an analgesic, tonic, antidiabetic, antihypertensive, anti-inflammatory, antitumor, and immune-stimulating agent. In this work, we carried out a comprehensive investigation into the anthocyanin components in the fruits of A. sessiliflorus. The anthocyanin content in the fresh fruits of A. sessiliflorus was determined by high performance liquid chromatography-diode array detection (HPLC/DAD), and the anthocyanin component was isolated from these using high-speed counter-current chromatography (HSCCC) and elucidated by electro-spray ionization-mass spectrometry (ESI/MS), 1H- and 13C-NMR. Its antioxidant activity was evaluated by ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). We found that A. sessiliflorus contained a gross anthocyanin content of 121.35 mg/100 g. HSCCC was successfully used for separation and purification of the primary anthocyanin component, cyanidin 3-xylosyl-galactoside. The antioxidant and radical scavenging tests indicated that cyanidin 3-xylosyl-galactoside is a potent antioxidant.

Highlights

  • Acanthopanax sessiliflorus (Rupr. & Maxim.) Seem. (Araliaceae) (Figure 1) is one of the most abundant species of genus Acanthopanax, which is a perennial plant native to the northeast region of China, Japan, and Korea

  • Of of total peak area and other anthocyanin components were present at trace levels only

  • We identified the principle anthocyanin in Acanthopanax sessiliflorus (Rupr. & Maxim.) Seem as

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Summary

Introduction

Acanthopanax sessiliflorus (Rupr. & Maxim.) Seem. (Araliaceae) (Figure 1) is one of the most abundant species of genus Acanthopanax, which is a perennial plant native to the northeast region of China, Japan, and Korea. (Araliaceae) (Figure 1) is one of the most abundant species of genus Acanthopanax, which is a perennial plant native to the northeast region of China, Japan, and Korea. The roots, stems, and leaves of A. sessiliflorus elicit a range of biological functions, including antipancreatic lipase, antiplatelet aggregation, and anti-inflammatory effects [1,2,3,4]. Various parts from the plant are used in a wide range of traditional medical applications, including the roots, shoots, bark, leaves, and fruits [2,5,6,7,8,9,10]. The fruits of A. sessiliflorus are used in traditional medical protocols as an analgesic, tonic, antidiabetic, antihypertensive, anti-inflammatory, antitumor and immune-stimulating agent [5,6,7].

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