Abstract
The aim of this research was to isolate bacteria capable of biotransforming daidzein from fresh feces from pregnant horses. A Hungate anaerobic roller tube was used for anaerobic culture. Single colonies were picked at random and incubated with daidzein. High performance liquid chromatography was used to detect whether the isolated bacteria were able to biotransform the substrate. A strain capable of reducing daidzein was selected and characterized using sequence analysis of 16S rDNA, and a phylogenetic tree was constructed. The morphological physiological and biochemical characteristics of the strain were investigated. A facultative anaerobic, Gram-positive bacterium capable of converting daidzein to dihydrodaidzein was isolated and named HXBM408 (MF992210). A BLAST search of HXBM408's 16S rDNA sequence against the GenBank database suggested that the strain has 99% similarity with Pediococcus acidilactici strain DSM (NR042057). The morphological, physiological, and biochemical characteristics of HXBM408 are very similar to those of Pediococcus. Based on these characteristics, the strain was identified as Pediococcus acidilactici. The bacterial strain HXBM408 isolated from the feces of pregnant horses was able to reduce the isoflavone daidzein to dihydrodaidzein.
Highlights
Dihydro daidzein (DHD) is a metabolite formed by daidzein in the animal intestines through the specific microbial group action
The bacteria were cultured on an anaerobic roller tube for two days, after which 86 bacterial colonies were isolated and each colony inoculated into 5 mL of Brain-heart infusion (BHI) medium, and incubated under anoxic conditions
Comparing the uniformity of the single strains after culture with the standard curves of daidzein and DHD, only one strain could be identified as producing DHD as a daidzein metabolite using HPLC
Summary
Dihydro daidzein (DHD) is a metabolite formed by daidzein in the animal intestines through the specific microbial group action. It has higher biological activity than its predecessor of the daidzein, mainly in estrogen-like form. It can be decomposed to produce Dihydodaidzein (DHD), Tihydodaidzein (THD), Equol, and O-desmethylangolensin (O-Dma), etc[3].DHD and THD have higher biological activity than daidzein. Chin-Dusting and colleagues have found that the ability of DHD and THD to regulate blood vessels is much higher than that of the precursor substance, daidzein[4]. Liang and colleagues studied the free radical scavenging activity of Daidzein, DHD, and O-DMA. The scavenging effect of DHD and O-DMA on free radicals
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