Isolation and Identification of Alternaria arborscens from corn grains and possibility controlling of it by using Agaricus bisporus filterate and sodium citrate and their toxin

Abstract

This study included isolating the accompanying and contaminated fungi of Corn Grains in the city of Al- Diwaniyah collected for the period September-December / 2021. A number of fungi were identified: of it Aternaria arborscens., It was observed that the percentage of surface fungi associated with non-sterile Corn Grains samples was high. Fungal toxins were detected in the fungal leachates of one of these fungus A. arborescenswas diagnosed with phenotype and genetically using PCR technology. So this species we have provided GenBank accession number(s) for it nucleotide sequence(s): ON386194 and ON386195 was recorded in Iraq in this study. The analysis was performed using HPLC technology showed results on the ability of A. arborescens to produce toxins. The highest concentration was the altratoxin which was 247.9 μg / ml. In the study Agaricus bisporus was used to conduct the biological resistance of pathogenic fungi to reduce the number and its toxicity of fungi A. arborescens. It is gave high resistance to A. arborescens when testing the contrast between them. The results of the study showed their ability to prevent the growth of pathogenic fungi in solid and liquid media.