Abstract

Alectrol, a germination stimulant for root parasitic plants, was purified from root exudates of red clover ( Trifolium pratense L.) and identified as a strigolactone, (+)-orobanchyl acetate [(3a S,4 S,8b S, E)-8,8-dimethyl-3-((( R)-4-methyl-5-oxo-2,5-dihydrofuran-2-yloxy)methylene)-2-oxo-3,3a,4,5,6,7,8,8b-octahydro-2 H-indeno[1,2- b]furan-4-yl acetate], by 1D and 2D NMR spectroscopy and ESI- and EI-MS spectrometry. Orobanchyl acetate afforded an [M–42] + ion in EI-MS and thus had been recognized as an isomer of strigol. Orobanchyl acetate was detected in root exudates of soybean ( Glycine max L.) and cowpea ( Vigina unguiculata L.) along with orobanchol.

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