Abstract
Agrobacterium rhizogenes is a common tool for transforming foreign genes into plant cell and inducing hairy root formation. A total of 274 colonies of Agrobacterium species was isolated on selective MG-Te medium from 235 samples of rhizophere soils. There were only thirteen colonies belonged to A. rhizogenes being able to efficiently induce the hairy root formation of Catharanthus roseus in vivo based on the morphological, biochemical, and pathogenicity tests. These thirteen colonies were identified as A. rhizogenes based on 16S rRNA sequence and possessing rolABC genes. The results indicated A. rhizogenes was universal in rhizophere soil in Vietnam and riched transgenic tools for researching C. roseus hairy roots in the future.
Highlights
A total of 274 colonies identified as A. rhizogenes based on 16S rRNA of Agrobacterium species was isolated on selective sequence and possessing rolABC genes
The results MG-Te medium from 235 samples of rhizophere soils. indicated A. rhizogenes was universal in rhizophere There were only thirteen colonies belonged to A. soil in Vietnam and riched transgenic tools for rhizogenes being able to efficiently induce the hairy researching C. roseus hairy roots in the future. root formation of Catharanthus roseus in vivo based
Trong số ba mươi bốn dòng phân lập được, qua kiểm tra bệnh học và kết quả phân tích sinh học phân tử cho thấy có mười ba chủng A. rhizogenes khác nhau về hình dạng khuẩn lạc cũng như khả năng cảm ứng tạo rễ tơ trên các giống dừa cạn in vivo
Summary
Trong đó có mười ba dòng có các đặc điểm hình thái, sinh hóa và bệnh học thuộc loài A. rhizogenes và có khả năng cảm ứng tạo thành rễ tơ trên cây dừa cạn in vivo. Cây đậu đen và cây Dừa cạn in vivo được sử dụng làm thực vật chủ để nhận dạng và sàng lọc chủng vi khuẩn được phân lập có khả năng gây cảm ứng tạo rễ tơ.
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